Composite

Part:BBa_K3782023:Design

Designed by: Jana Näf   Group: iGEM21_UNILausanne   (2021-10-20)


cspA-RBS-TEE-His-RiAFP-Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 394
    Illegal AgeI site found at 427
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for optimal expression in Escherichia coli.


Source

The source of RiAFP is the Siberian cerambycid beetle Rhagium inquisitor. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/HQ540314.1 The part was synthesized with a 6x His tag.

References